The authors successfully generated pulsed molecular beams with high repetition rate by using a cantilever piezo valve, and the highest repetition rate could reach 3 kHz. The interaction between intense femtosecond laser fields and nitrogen molecular beams were studied, in which the molecular beams were produced by cantilever piezo valve. Strong fluorescence emissions, which could be assigned to N2 +(B-X), were observed. The performance of pulse valve was characterized based on the measurement of the fluorescence emission as a function of the repetition rate and the time delay of the pulsed valve. The results demonstrate that pulsed molecular beams can be generated with high quality and high repetition rate. The combination of such pulsed molecular beams and high-frequency kHz femtosecond lasers will promote the experimental study of molecular dynamics driven by intense femtosecond laser fields.